Hotspot

rps19-rpl2

IRb · 84271-84325 · 55 bp

Back to Eschenbachia

Pi 0.0189

MarkerSeek score 65.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 55
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CGCGGGACCAGGTTATTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	57.8	58.1	189-191 bp	1.0	78.5
rps19-rpl2_p2	CGCGGGACCAGGTTATTATT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.8	58.1	281-283 bp	1.0	78.4
rps19-rpl2_p3	CGCGGGACCAGGTTATTATT	CACAACCCCTTGGGGTTATC	50.0	55.0	57.8	58.2	256-258 bp	1.0	77.7
rps19-rpl2_p4	CGCGGGACCAGGTTATTATT	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.8	57.8	247-249 bp	1.0	77.3
rps19-rpl2_p5	CGCGGGACCAGGTTATTATT	ATTTGATTCTTCGTCGCCGT	50.0	45.0	57.8	58.3	191-193 bp	1.0	77.0