Hotspot

ycf4-cemA

LSC · 60545-61448 · 904 bp

Back to Eschenbachia

Pi 0.00906

MarkerSeek score 66.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 904
variable_sites: 8
pi: 0.00906
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1084-1085 bp	1.0	81.3
ycf4-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1048-1049 bp	1.0	80.8
ycf4-cemA_p3	GAGCCATTCCATTGACTCGT	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.0	57.6	1083-1084 bp	1.0	78.7
ycf4-cemA_p4	CTCCACGGGAAATGGAACAA	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.1	57.6	1047-1048 bp	1.0	78.3
ycf4-cemA_p5	GGAGCCATTCCATTGACTCG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.7	58.1	1085-1086 bp	1.0	76.0