Hotspot

trnF-GAA-ndhJ

LSC · 48291-48934 · 644 bp

Back to Eschenbachia

Pi 0.014

MarkerSeek score 66.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 9
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	747-752 bp	1.0	70.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	746-751 bp	1.0	70.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	754-759 bp	1.0	70.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	50.0	47.6	58.1	57.5	748-753 bp	1.0	68.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	50.0	47.6	58.1	58.6	747-752 bp	1.0	67.8