Hotspot

trnG-trnT-GGU

LSC · 30830-31247 · 418 bp

Back to Eschenbachia

Pi 0.0443

MarkerSeek score 75.00

Primer available yes

Label trnG-trnT-GGU

Feature Metadata

label_name: trnG-trnT-GGU
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 14
pi: 0.0443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnT-GGU_p1	AATGAAACCGAAAGACCCCT	TCCCCTATCGGATCTGAACC	45.0	55.0	57.0	58.0	509-561 bp	1.0	78.2
trnG-trnT-GGU_p2	ATGAAACCGAAAGACCCCTT	TCCCCTATCGGATCTGAACC	45.0	55.0	57.0	58.0	508-560 bp	1.0	78.2
trnG-trnT-GGU_p3	AATGAAACCGAAAGACCCCT	GATCTGAACCGAGGGCTTAC	45.0	55.0	57.0	57.8	499-551 bp	1.0	76.5
trnG-trnT-GGU_p4	ATGAAACCGAAAGACCCCTT	GATCTGAACCGAGGGCTTAC	45.0	55.0	57.0	57.8	498-550 bp	1.0	76.5
trnG-trnT-GGU_p5	GAATGAAACCGAAAGACCCCT	TCCCCTATCGGATCTGAACC	47.6	55.0	58.2	58.0	510-562 bp	1.0	76.3