Hotspot

psbM-trnD-GUC

LSC · 10642-11255 · 614 bp

Back to Eschenbachia

Pi 0.0183

MarkerSeek score 67.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 11
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAGGCAGTAGGAACT	TTCAATAGGCTAGAGCACCG	42.9	50.0	55.7	57.1	733-746 bp	1.0	49.3
psbM-trnD-GUC_p2	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.6	57.1	718-731 bp	1.0	48.1
psbM-trnD-GUC_p3	AGTAGAAAGGCAGTAGGAACT	CAATAGGCTAGAGCACCGC	42.9	57.9	55.7	58.1	731-744 bp	1.0	47.7
psbM-trnD-GUC_p4	AAAATGAACAGTGCAGTAGC	TTCAATAGGCTAGAGCACCG	40.0	50.0	54.5	57.1	712-725 bp	1.0	47.7
psbM-trnD-GUC_p5	AAGTAGAAAGGCAGTAGGAACT	TTCAATAGGCTAGAGCACCG	40.9	50.0	56.5	57.1	734-747 bp	1.0	47.4