Hotspot

ycf1

SSC · 125484-130883 · 5400 bp

Back to Erythrostemon

Pi 0.0259

MarkerSeek score 52.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5400
variable_sites: 139
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACGATAGGGTCCGTTCAAG	GCTCGGGTTATGGAAGAAGG	55.0	55.0	58.1	58.0	1210-1213 bp	1.0	80.9
ycf1_p2	GAGAGCTTCGGCCCTATTTC	GCTCGGGTTATGGAAGAAGG	55.0	55.0	58.1	58.0	1092-1095 bp	1.0	80.6
ycf1_p3	CACGATAGGGTCCGTTCAAG	AGGGCCCTCTTATCTCTTCC	55.0	55.0	58.1	57.9	1238-1241 bp	1.0	80.4
ycf1_p4	CACGATAGGGTCCGTTCAAG	GGGCCCTCTTATCTCTTCCT	55.0	55.0	58.1	57.9	1237-1240 bp	1.0	80.4
ycf1_p5	ACGATAGGGTCCGTTCAAGA	GCTCGGGTTATGGAAGAAGG	50.0	55.0	58.2	58.0	1209-1212 bp	1.0	80.3