Hotspot

rpl16

LSC · 82858-84347 · 1490 bp

Back to Erythrostemon

Pi 0.029

MarkerSeek score 64.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1490
variable_sites: 43
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCATCCCAAAGACCTCTTTCC	TGATTTGATATTGAGATAATTGCT	47.6	25.0	57.3	52.6	1545-1549 bp	1.0	42.6
rpl16_p2	CATCCCAAAGACCTCTTTCCT	TGATTTGATATTGAGATAATTGCT	47.6	25.0	57.3	52.6	1544-1548 bp	1.0	42.6
rpl16_p3	TTCATCCCAAAGACCTCTTTCC	TGATTTGATATTGAGATAATTGCT	45.5	25.0	58.0	52.6	1546-1550 bp	1.0	42.6
rpl16_p4	CATCCCAAAGACCTCTTTCCTT	TGATTTGATATTGAGATAATTGCT	45.5	25.0	58.0	52.6	1544-1548 bp	1.0	42.6
rpl16_p5	TCCCAAAGACCTCTTTCCTT	TGATTTGATATTGAGATAATTGCT	45.0	25.0	55.9	52.6	1542-1546 bp	1.0	42.6