Hotspot

ndhD-psaC

SSC · 118644-118768 · 125 bp

Back to Erythrostemon

Pi 0.0732

MarkerSeek score 78.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 125
variable_sites: 9
pi: 0.0732
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAAGATTAAGGAACCCGCG	AAACAACTCGAAGCATGGGT	50.0	45.0	57.7	58.0	222-224 bp	1.0	81.3
ndhD-psaC_p2	AGAAGATTAAGGAACCCGCG	GGCATGAAACAACTCGAAGC	50.0	50.0	57.7	58.0	228-230 bp	1.0	81.1
ndhD-psaC_p3	TAAGGAACCCGCGGATATTG	AAACAACTCGAAGCATGGGT	50.0	45.0	57.5	58.0	215-217 bp	1.0	79.6
ndhD-psaC_p4	TAAGGAACCCGCGGATATTG	GGCATGAAACAACTCGAAGC	50.0	50.0	57.5	58.0	221-223 bp	1.0	79.4
ndhD-psaC_p5	AGAAGATTAAGGAACCCGCG	CAGAGGACTGTGTTGGTTGT	50.0	50.0	57.7	57.7	303-305 bp	1.0	77.7