Hotspot

ndhF-rpl32

SSC · 114258-115180 · 923 bp

Back to Erythrostemon

Pi 0.0328

MarkerSeek score 68.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 923
variable_sites: 29
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAATATTGATGTGTATGTTCCA	GCTGCCCAATATCCCTTCTC	31.8	55.0	52.0	58.1	999-1029 bp	1.0	45.4
ndhF-rpl32_p2	GAATATTGATGTGTATGTTCCA	TGCCCAATATCCCTTCTCCT	31.8	50.0	52.0	57.8	997-1027 bp	1.0	45.4
ndhF-rpl32_p3	GAATATTGATGTGTATGTTCCA	TCAATGCTGCCCAATATCCC	31.8	50.0	52.0	58.0	1004-1034 bp	1.0	45.3
ndhF-rpl32_p4	GAATATTGATGTGTATGTTCCA	CAATGCTGCCCAATATCCCT	31.8	50.0	52.0	58.0	1003-1033 bp	1.0	45.3
ndhF-rpl32_p5	GAATATTGATGTGTATGTTCCA	AAAAGCTTTCAATGCTGCCC	31.8	45.0	52.0	57.8	1012-1042 bp	1.0	45.3