Hotspot

trnW-CCA-trnP-UGG

LSC · 67766-67990 · 225 bp

Back to Erythrostemon

Pi 0.0734

MarkerSeek score 77.70

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 16
pi: 0.0734
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	276-279 bp	1.0	85.3
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	278-281 bp	1.0	85.2
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	279-282 bp	1.0	85.2
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	289-292 bp	1.0	85.0
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	290-293 bp	1.0	85.0