Hotspot

ndhC-trnV-UAC

LSC · 52377-52886 · 510 bp

Back to Erythrostemon

Pi 0.0627
MarkerSeek score 71.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
510
variable_sites
23
pi
0.0627
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 513-653 bp 1.0 82.8
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 512-652 bp 1.0 80.1
ndhC-trnV-UAC_p3 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 520-660 bp 1.0 80.0
ndhC-trnV-UAC_p4 TCTCTGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 521-661 bp 1.0 79.8
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 501-641 bp 1.0 79.0