Hotspot

psbZ-trnG-GCC

LSC · 37524-37973 · 450 bp

Back to Erythrostemon

Pi 0.0742
MarkerSeek score 86.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
450
variable_sites
32
pi
0.0742
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.0 57.7 605-616 bp 1.0 84.0
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 608-619 bp 1.0 82.4
psbZ-trnG-GCC_p3 TCCTGATGGTTGGTCGAGTA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 601-612 bp 1.0 82.3
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.3 57.7 606-617 bp 1.0 82.0
psbZ-trnG-GCC_p5 TTCTCCTGATGGTTGGTCGA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 58.4 57.7 604-615 bp 1.0 81.2