Pi
0.0588
MarkerSeek score
73.40
Primer available
yes
Label
rps2-rpoC2
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rps2-rpoC2_p1 | CCATGGCCAAAATGAACTCC | TCAGAGCAATCATTTCGAGGA | 50.0 | 42.9 | 57.3 | 57.1 | 325-330 bp | 1.0 | 62.6 |
| rps2-rpoC2_p2 | GGCCAAAATGAACTCCTGCT | TCAGAGCAATCATTTCGAGGA | 50.0 | 42.9 | 58.7 | 57.1 | 321-326 bp | 1.0 | 62.2 |
| rps2-rpoC2_p3 | TGGCCAAAATGAACTCCTGC | TCAGAGCAATCATTTCGAGGA | 50.0 | 42.9 | 59.0 | 57.1 | 322-327 bp | 1.0 | 59.8 |
| rps2-rpoC2_p4 | GCCAAAATGAACTCCTGCTTC | TCAGAGCAATCATTTCGAGGA | 47.6 | 42.9 | 58.0 | 57.1 | 320-325 bp | 1.0 | 59.7 |
| rps2-rpoC2_p5 | GGTGCCATTCTAGGATTCCA | TCAGAGCAATCATTTCGAGGA | 50.0 | 42.9 | 57.0 | 57.1 | 355-360 bp | 1.0 | 59.5 |