Hotspot

ndhG-ndhI

SSC · 124087-124543 · 457 bp

Back to Erythrophleum

Pi 0.0387

MarkerSeek score 76.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 457
variable_sites: 25
pi: 0.0387
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACAAGACCTGATCCCAGAAAG	AATCAAATTGCTTTGGGCCG	47.6	45.0	57.3	57.9	556-595 bp	1.0	73.0
ndhG-ndhI_p2	ACAAGACCTGATCCCAGAAAG	TTTGGGCCGTTTACCAATGT	47.6	45.0	57.3	58.3	545-584 bp	1.0	71.5
ndhG-ndhI_p3	ACAAGACCTGATCCCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	56.0	57.9	556-595 bp	1.0	71.1
ndhG-ndhI_p4	ACAAGACCTGATCCCAGAAAG	CTTTGGGCCGTTTACCAATG	47.6	50.0	57.3	57.6	546-585 bp	1.0	70.9
ndhG-ndhI_p5	CAAGACCTGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	47.6	45.0	57.0	57.9	555-594 bp	1.0	70.8