Hotspot

ndhF-rpl32

SSC · 117914-118849 · 936 bp

Back to Erythrophleum

Pi 0.016

MarkerSeek score 64.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 936
variable_sites: 25
pi: 0.016
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTGGGAAAAAGGAGAAGT	TCAATGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1111-1118 bp	1.0	80.3
ndhF-rpl32_p2	CCGTGGGAAAAAGGAGAAGT	CAATGCTGCCCAATATCCCT	50.0	50.0	57.7	58.0	1110-1117 bp	1.0	80.2
ndhF-rpl32_p3	TTTGTTGCCGTGGGAAAAAG	TCAATGCTGCCCAATATCCC	45.0	50.0	57.7	58.0	1118-1125 bp	1.0	80.0
ndhF-rpl32_p4	TTTGTTGCCGTGGGAAAAAG	CAATGCTGCCCAATATCCCT	45.0	50.0	57.7	58.0	1117-1124 bp	1.0	80.0
ndhF-rpl32_p5	CCGTGGGAAAAAGGAGAAGT	GCTGCCCAATATCCCTTCTC	50.0	55.0	57.7	58.1	1106-1113 bp	1.0	79.5