Hotspot

rps8-rpl14

LSC · 85598-85827 · 230 bp

Back to Erythrophleum

Pi 0.0348

MarkerSeek score 65.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 12
pi: 0.0348
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GCAATGGTGTCCTTACCCAT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.9	57.9	251-325 bp	1.0	85.4
rps8-rpl14_p2	GATGGTATTCGAACCGTCCC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.1	57.9	314-388 bp	1.0	84.8
rps8-rpl14_p3	GCAATGGTGTCCTTACCCAT	AGTTTCATTAGCACCCGAGG	50.0	50.0	57.9	57.6	252-326 bp	1.0	83.0
rps8-rpl14_p4	GGTATTCGAACCGTCCCTTT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.6	57.9	311-385 bp	1.0	82.8
rps8-rpl14_p5	GATGGTATTCGAACCGTCCC	AGTTTCATTAGCACCCGAGG	55.0	50.0	58.1	57.6	315-389 bp	1.0	82.5