Hotspot

petD-rpoA

LSC · 82576-82790 · 215 bp

Back to Erythrophleum

Pi 0.0159

MarkerSeek score 64.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 5
pi: 0.0159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	TCGCATAGAAGATGTAAAACGT	50.0	36.4	58.1	55.9	327-335 bp	1.0	50.1
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	TCGCATAGAAGATGTAAAACGT	50.0	36.4	58.1	55.9	328-336 bp	1.0	50.0
petD-rpoA_p3	CCCATTTCGTCGTCCAGTAG	TCGCATAGAAGATGTAAAACGT	55.0	36.4	58.1	55.9	386-394 bp	1.0	49.6
petD-rpoA_p4	GGTTGGGTATTGGAGCAACA	TGGAGCTTTTGAATAACAGTCA	50.0	36.4	58.1	55.9	376-384 bp	1.0	49.5
petD-rpoA_p5	CCCATTTCGTCGTCCAGTAG	TGGAGCTTTTGAATAACAGTCA	55.0	36.4	58.1	55.9	435-443 bp	1.0	49.2