Hotspot

petA-psbJ

LSC · 67192-68360 · 1169 bp

Back to Erythrophleum

Pi 0.0153

MarkerSeek score 70.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1169
variable_sites: 31
pi: 0.0153
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1270-1332 bp	1.0	79.2
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1296-1358 bp	1.0	77.8
petA-psbJ_p3	TGTTCTTCTTGGCATCCGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1280-1342 bp	1.0	77.6
petA-psbJ_p4	TTGTTCTTCTTGGCATCCGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1281-1343 bp	1.0	77.6
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1301-1363 bp	1.0	77.3