Hotspot

ycf4-cemA

LSC · 64864-65318 · 455 bp

Back to Erythrophleum

Pi 0.042
MarkerSeek score 74.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
455
variable_sites
28
pi
0.042
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT AAGGGGAATGAATGCTTTCT 45.0 40.0 58.1 54.9 493-524 bp 1.0 62.6
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT AGGGGAATGAATGCTTTCTT 45.0 40.0 58.1 54.9 492-523 bp 1.0 62.6
ycf4-cemA_p3 GAACAAAAAGCGGCGGAATT AAGGGGAATGAATGCTTTCT 45.0 40.0 58.2 54.9 511-542 bp 1.0 61.4
ycf4-cemA_p4 GAACAAAAAGCGGCGGAATT AGGGGAATGAATGCTTTCTT 45.0 40.0 58.2 54.9 510-541 bp 1.0 61.4
ycf4-cemA_p5 TTGGCCTATTTCTTGCGTGT AGGGGAATGAATGCTTTCTTT 45.0 38.1 58.1 55.7 492-523 bp 1.0 61.3