Hotspot

trnT-UGU-trnL-UAA

LSC · 50171-51620 · 1450 bp

Back to Erythrophleum

Pi 0.00976
MarkerSeek score 68.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1450
variable_sites
23
pi
0.00976
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1350-1532 bp 1.0 83.3
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1358-1540 bp 1.0 82.8
trnT-UGU-trnL-UAA_p3 AAATGCGACGCTCTAACCTC ACCAATTTCGCCATATCCCC 50.0 50.0 58.4 58.0 1322-1504 bp 1.0 82.4
trnT-UGU-trnL-UAA_p4 AAATGCGACGCTCTAACCTC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.4 58.1 1330-1512 bp 1.0 81.9
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 1364-1546 bp 1.0 81.4