Hotspot

atpH-atpI

LSC · 15109-16503 · 1395 bp

Back to Erythrophleum

Pi 0.0135

MarkerSeek score 73.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1395
variable_sites: 33
pi: 0.0135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.0	57.9	1429-1481 bp	1.0	84.2
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.2	57.9	1430-1482 bp	1.0	83.0
atpH-atpI_p3	CCTTCTACAGCTTGACCTGC	TTTGCAACTTTAGCTGCAGC	55.0	45.0	58.3	57.9	1510-1562 bp	1.0	82.1
atpH-atpI_p4	TCCCTTCTACAGCTTGACCT	TTTGCAACTTTAGCTGCAGC	50.0	45.0	57.7	57.9	1512-1564 bp	1.0	81.6
atpH-atpI_p5	CCCTTCTACAGCTTGACCTG	TTTGCAACTTTAGCTGCAGC	55.0	45.0	57.6	57.9	1511-1563 bp	1.0	81.1