Hotspot

trnR-UCU-atpA

LSC · 11199-11609 · 411 bp

Back to Erythrophleum

Pi 0.0359

MarkerSeek score 72.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 25
pi: 0.0359
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CGGGAAGCTGAAGTCCTTTT	47.6	50.0	57.3	58.1	517-564 bp	1.0	73.2
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CATTCACTCGGGAAGCTGAA	47.6	50.0	57.3	57.9	525-572 bp	1.0	73.1
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	47.6	50.0	57.3	57.9	529-576 bp	1.0	73.1
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTCACTCGGGAAGCTGAAG	47.6	50.0	57.3	57.6	524-571 bp	1.0	70.8
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	47.6	50.0	57.3	57.5	531-578 bp	1.0	70.1