Hotspot

ndhE-ndhG

SSC · 116086-116276 · 191 bp

Back to Erythronium

Pi 0.0377

MarkerSeek score 62.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 191
variable_sites: 19
pi: 0.0377
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	CTAGCCCAATAGCAGCTTCA	ATTGGTTGAATGGGCCCAAT	50.0	45.0	57.4	58.0	1124-1140 bp	1.0	80.1
ndhE-ndhG_p2	TAGCCCAATAGCAGCTTCAG	ATTGGTTGAATGGGCCCAAT	50.0	45.0	57.4	58.0	1123-1139 bp	1.0	80.1
ndhE-ndhG_p3	ATAGACACCAGGACACCAGA	ATTGGTTGAATGGGCCCAAT	50.0	45.0	57.4	58.0	1510-1536 bp	0.909091	76.8
ndhE-ndhG_p4	ACACCAGGACACCAGATGAA	ATTGGTTGAATGGGCCCAAT	50.0	45.0	58.6	58.0	1526-1532 bp	0.181818	43.5
ndhE-ndhG_p5	TAGACACCAGGACACCAGAT	ATTGGTTGAATGGGCCCAAT	50.0	45.0	57.4	58.0	1529-1535 bp	0.181818	43.0