Hotspot

ndhF-rpl32

SSC · 110590-111308 · 719 bp

Back to Erythronium

Pi 0.0233

MarkerSeek score 66.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 719
variable_sites: 48
pi: 0.0233
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCGTATTGATATGTTTGTTCCA	ACTGCCGTTAAATACCCCTTC	34.8	47.6	56.9	58.0	799-836 bp	1.0	48.9
ndhF-rpl32_p2	TGCGTATTGATATGTTTGTTCCA	ACTGCCGTTAAATACCCCTT	34.8	45.0	56.9	56.8	799-836 bp	1.0	48.9
ndhF-rpl32_p3	TCCATGCGTATTGATATGTTTGT	ACTGCCGTTAAATACCCCTTC	34.8	47.6	56.7	58.0	803-840 bp	1.0	48.9
ndhF-rpl32_p4	TGCGTATTGATATGTTTGTTCCA	CTGCCGTTAAATACCCCTTCT	34.8	47.6	56.9	57.7	798-835 bp	1.0	48.9
ndhF-rpl32_p5	TGCGTATTGATATGTTTGTTCCA	AGAAAGAGCTTTTACTGCCGT	34.8	42.9	56.9	57.9	812-849 bp	1.0	48.8