Hotspot

trnN-GUU-ndhF

IRb · 106494-108363 · 1870 bp

Back to Erythronium

Pi 0.0076

MarkerSeek score 40.20

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1870
variable_sites: 29
pi: 0.0076
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TGGATCTACGCCGAGGTATT	AGTTGGGGTTGCAAGTTTCT	50.0	45.0	58.0	57.8	1933-2255 bp	0.454545	61.9
trnN-GUU-ndhF_p2	TGGATCTACGCCGAGGTATT	AAATGGAGTTGGGGTTGCAA	50.0	45.0	58.0	58.2	1939-2261 bp	0.454545	61.6
trnN-GUU-ndhF_p3	CGGGAGAATTGATGACTGCA	AGTTGGGGTTGCAAGTTTCT	50.0	45.0	58.0	57.8	2039-2361 bp	0.181818	50.0
trnN-GUU-ndhF_p4	CATGATCGGGATAGCAGACG	AGTTGGGGTTGCAAGTTTCT	55.0	45.0	58.0	57.8	2619 bp	0.090909	42.3
trnN-GUU-ndhF_p5	CATGATCGGGATAGCAGACG	AAATGGAGTTGGGGTTGCAA	55.0	45.0	58.0	58.2	2625 bp	0.090909	41.9