Hotspot

rps18-rpl20

LSC · 66896-67163 · 268 bp

Back to Erythronium

Pi 0.0365

MarkerSeek score 70.80

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 268
variable_sites: 21
pi: 0.0365
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AGCCGAATCGATCCCTAGAA	TGTACAAGGGGCAATTGCTT	50.0	45.0	58.0	58.0	401-418 bp	1.0	88.4
rps18-rpl20_p2	GCCGAATCGATCCCTAGAAC	TGTACAAGGGGCAATTGCTT	55.0	45.0	57.9	58.0	400-417 bp	1.0	87.9
rps18-rpl20_p3	AGCCGAATCGATCCCTAGAA	CACAATCTGTACAAGGGGCA	50.0	50.0	58.0	57.8	408-425 bp	1.0	86.8
rps18-rpl20_p4	AGCCGAATCGATCCCTAGAA	ACACAATCTGTACAAGGGGC	50.0	50.0	58.0	57.8	409-426 bp	1.0	86.8
rps18-rpl20_p5	ATCCCTAGAACTGTGGGTCC	TGTACAAGGGGCAATTGCTT	55.0	45.0	58.1	58.0	391-408 bp	0.909091	83.4