Hotspot

trnP-UGG-psaJ

LSC · 65035-65439 · 405 bp

Back to Erythronium

Pi 0.0244

MarkerSeek score 67.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 33
pi: 0.0244
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	517-623 bp	1.0	87.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	516-622 bp	1.0	87.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	528-634 bp	1.0	87.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	527-633 bp	1.0	87.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	530-636 bp	1.0	87.2