Hotspot

accD-psaI

LSC · 57395-58205 · 811 bp

Back to Erythronium

Pi 0.0211

MarkerSeek score 67.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 51
pi: 0.0211
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1010-1058 bp	0.909091	83.3
accD-psaI_p2	TCACAAGCGGCTGAGTATTC	TTCCACGCAATTGTACACCA	50.0	45.0	58.0	58.0	1599-1651 bp	0.909091	81.0
accD-psaI_p3	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1249 bp	0.090909	43.4
accD-psaI_p4	GCATGCAAGAAGGAAGCTTG	TTCCACGCAATTGTACACCA	50.0	45.0	58.0	58.0	1838 bp	0.090909	43.3
accD-psaI_p5	GCATGCAAGAAGGAAGCTTG	CCCGCGATCCCATAAAAAGA	50.0	50.0	58.0	58.0	1796 bp	0.090909	43.3