Hotspot

rbcL-accD

LSC · 55013-55915 · 903 bp

Back to Erythronium

Pi 0.0151

MarkerSeek score 64.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 43
pi: 0.0151
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TCGAGTTCGAACCGGTAGAT	CAACATCGAATTGAACCACCA	50.0	42.9	58.3	57.1	962-1127 bp	1.0	70.1
rbcL-accD_p2	TCGAGTTCGAACCGGTAGAT	ACAACATCGAATTGAACCACC	50.0	42.9	58.3	57.1	963-1128 bp	1.0	70.1
rbcL-accD_p3	TCGAGTTCGAACCGGTAGAT	CCACTGATTTACTTAGCCCACA	50.0	45.5	58.3	58.0	1008-1173 bp	1.0	68.9
rbcL-accD_p4	TCGAGTTCGAACCGGTAGAT	TCCACTGATTTACTTAGCCCAC	50.0	45.5	58.3	57.8	1009-1174 bp	1.0	67.5
rbcL-accD_p5	TCGAGTTCGAACCGGTAGAT	AACAACATCGAATTGAACCACC	50.0	40.9	58.3	57.8	964-1129 bp	1.0	67.5