Hotspot

ndhC-trnV-UAC

LSC · 48779-49761 · 983 bp

Back to Erythronium

Pi 0.0176

MarkerSeek score 70.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 50
pi: 0.0176
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TTCCGGCCCTTCACTAATTG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1108-1168 bp	1.0	84.3
ndhC-trnV-UAC_p2	GCCCTTCACTAATTGGTGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1103-1163 bp	1.0	84.1
ndhC-trnV-UAC_p3	CTTTTCCGGCCCTTCACTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1111-1171 bp	1.0	83.7
ndhC-trnV-UAC_p4	TTCCGGCCCTTCACTAATTG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1107-1167 bp	1.0	81.6
ndhC-trnV-UAC_p5	GCCCTTCACTAATTGGTGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.2	57.5	1102-1162 bp	1.0	81.3