Hotspot

trnT-UGU-trnL-UAA

LSC · 44762-45519 · 758 bp

Back to Erythronium

Pi 0.0167

MarkerSeek score 65.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 40
pi: 0.0167
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	796-833 bp	1.0	84.9
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	828-865 bp	1.0	83.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	790-827 bp	1.0	83.6
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	828-865 bp	1.0	82.7
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CAGGTTTCCATACCAAGGCT	55.0	50.0	57.9	57.5	830-867 bp	1.0	82.6