Hotspot

trnS-GGA-rps4

LSC · 43435-43739 · 305 bp

Back to Erythronium

Pi 0.0172

MarkerSeek score 64.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 15
pi: 0.0172
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	ATTATTCCCGTCAGACTTGA	50.0	40.0	58.1	53.8	353-365 bp	1.0	51.8
trnS-GGA-rps4_p2	CACTCGGTTTCTGGCCAATA	ATTATTCCCGTCAGACTTGA	50.0	40.0	57.9	53.8	433-445 bp	1.0	50.5
trnS-GGA-rps4_p3	GGCTTTTGTTTACCGAGGGT	ATTATTCCCGTCAGACTTGA	50.0	40.0	58.4	53.8	352-364 bp	1.0	49.5
trnS-GGA-rps4_p4	TATGAAGGAGAGATGGCCGA	ATTATTCCCGTCAGACTTGA	50.0	40.0	57.6	53.8	406-418 bp	1.0	48.7
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	AGTCGTAGAATATTATTCCCGTCA	50.0	37.5	58.1	57.1	364-376 bp	1.0	46.4