Hotspot

trnE-UUC-trnT-GGU

LSC · 29352-30201 · 850 bp

Back to Erythronium

Pi 0.0223

MarkerSeek score 61.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 53
pi: 0.0223
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCATCGACCCAGGAAGAATT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	918-1169 bp	1.0	88.0
trnE-UUC-trnT-GGU_p2	CTTGCTTCCTGTCCCACTTT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1425-1682 bp	1.0	87.8
trnE-UUC-trnT-GGU_p3	GAACTACAATCCCAGCGAGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1297-1554 bp	1.0	87.3
trnE-UUC-trnT-GGU_p4	AATGGAGCGGCGAATTTTTG	GAACCGATGACTTACGCCTT	45.0	50.0	58.0	58.0	1111-1139 bp	0.181818	50.1
trnE-UUC-trnT-GGU_p5	CAATGGAGCGGCGAATTTTT	GAACCGATGACTTACGCCTT	45.0	50.0	58.0	58.0	1112-1140 bp	0.181818	50.1