Hotspot

psbM-trnD-GUC

LSC · 28256-28755 · 500 bp

Back to Erythronium

Pi 0.0111

MarkerSeek score 63.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 23
pi: 0.0111
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTGACTTCCA	CCTCGCTGGGATTGTAGTTC	40.0	55.0	54.5	58.1	572-602 bp	1.0	58.5
psbM-trnD-GUC_p2	TGCGAGAATATTGACTTCCA	TGTATGCCATAACCTCGCTG	40.0	50.0	54.5	57.8	584-614 bp	1.0	57.0
psbM-trnD-GUC_p3	TGCGAGAATATTGACTTCCA	CATAACCTCGCTGGGATTGT	40.0	50.0	54.5	57.7	577-607 bp	1.0	56.3
psbM-trnD-GUC_p4	TGCGAGAATATTGACTTCCA	CCATAACCTCGCTGGGATTG	40.0	55.0	54.5	58.4	578-608 bp	1.0	55.8
psbM-trnD-GUC_p5	TGCGAGAATATTGACTTCCAT	CCTCGCTGGGATTGTAGTTC	38.1	55.0	55.1	58.1	572-602 bp	1.0	55.5