Hotspot

rps16-trnQ-UUG

LSC · 5926-6791 · 866 bp

Back to Erythronium

Pi 0.0219
MarkerSeek score 68.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
866
variable_sites
53
pi
0.0219
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 909-939 bp 1.0 86.5
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 920-950 bp 1.0 85.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 913-943 bp 1.0 85.3
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 924-954 bp 1.0 84.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CGGTAAGGCAATGGGTTTTG 45.0 50.0 58.1 57.6 942-972 bp 1.0 84.0