Hotspot

ycf1

SSC · 123905-129367 · 5463 bp

Back to Erythrodes

Pi 0.00988

MarkerSeek score 50.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5463
variable_sites: 54
pi: 0.00988
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCTCCGACGTATGGAAGA	TCTATAAGAAGGGACGGAAA	50.0	40.0	57.3	52.8	227 bp	1.0	42.1
ycf1_p2	TCTCTCCGACGTATGGAAGA	TCTATCTATAAGAAGGGACGGA	50.0	40.9	57.3	54.7	231 bp	1.0	42.1
ycf1_p3	TCTCTCCGACGTATGGAAGA	TCTATAAGAAGGGACGGAAAA	50.0	38.1	57.3	53.7	227 bp	1.0	42.1
ycf1_p4	TCTCTCCGACGTATGGAAGA	ATCTATAAGAAGGGACGGAA	50.0	40.0	57.3	52.5	228 bp	1.0	42.1
ycf1_p5	TCTCTCCGACGTATGGAAGA	TCTATCTATAAGAAGGGACGGAA	50.0	39.1	57.3	55.5	231 bp	1.0	42.1