Hotspot

clpP-psbB

LSC · 71048-71699 · 652 bp

Back to Erythrodes

Pi 0.0156

MarkerSeek score 69.50

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 652
variable_sites: 10
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGGGGAAATACCGTATGACC	GGCCCAACCAGAAACTAGAG	55.0	55.0	57.8	57.9	866-874 bp	1.0	79.8
clpP-psbB_p2	ACCCAAACTGCATCTTCCTC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	816-824 bp	1.0	79.6
clpP-psbB_p3	CGGGGAAATACCGTATGACC	TGGACAGAAAGCAATCGACC	55.0	50.0	57.8	58.2	832-840 bp	1.0	79.3
clpP-psbB_p4	ATACGTCAACCCAAACTGCA	GGCCCAACCAGAAACTAGAG	45.0	55.0	57.7	57.9	824-832 bp	1.0	79.1
clpP-psbB_p5	TACGTCAACCCAAACTGCAT	GGCCCAACCAGAAACTAGAG	45.0	55.0	57.7	57.9	823-831 bp	1.0	79.1