Hotspot

accD-psaI

LSC · 57962-58759 · 798 bp

Back to Erythrodes

Pi 0.0113

MarkerSeek score 65.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 9
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCGGCTGAGTATTCATTCCA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	988-994 bp	1.0	80.0
accD-psaI_p2	GCGGCTGAGTATTCATTCCA	TGCAATTGCCGGAAAGACTA	50.0	45.0	58.0	57.8	978-984 bp	1.0	79.3
accD-psaI_p3	GCGGCTGAGTATTCATTCCA	CATTGCAATTGCCGGAAAGA	50.0	45.0	58.0	57.6	981-987 bp	1.0	77.9
accD-psaI_p4	GCGGCTGAGTATTCATTCCA	ATTGCAATTGCCGGAAAGAC	50.0	45.0	58.0	57.6	980-986 bp	1.0	77.9
accD-psaI_p5	GCGGCTGAGTATTCATTCCA	CGGAAAGACTAGGCCCACTA	50.0	55.0	58.0	58.5	969-975 bp	1.0	76.7