Hotspot

ndhC-trnV-UAC

LSC · 49861-50445 · 585 bp

Back to Erythrodes

Pi 0.0157

MarkerSeek score 66.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 9
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCGGAAATGACAAATGCCA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.1	57.8	705-759 bp	1.0	80.0
ndhC-trnV-UAC_p2	TCTGGTCCTTCCCTAATCGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	734-788 bp	1.0	78.9
ndhC-trnV-UAC_p3	AATCGGGGCTAAAATTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	720-774 bp	1.0	78.2
ndhC-trnV-UAC_p4	TCCGGAAATGACAAATGCCA	AGCCCAATGCAACAAAATGC	45.0	45.0	58.1	58.5	787-841 bp	1.0	77.6
ndhC-trnV-UAC_p5	TCCGGAAATGACAAATGCCA	GAAGGTCTACGGTTCGAGTC	45.0	55.0	58.1	57.5	704-758 bp	1.0	77.3