Hotspot

psaA-ycf3

LSC · 40865-41502 · 638 bp

Back to Erythrodes

Pi 0.00955

MarkerSeek score 65.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 6
pi: 0.00955
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ACAGGCTTGGTTCGATCAAG	50.0	50.0	57.9	58.2	829-842 bp	1.0	79.3
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GACAGGCTTGGTTCGATCAA	50.0	50.0	57.9	58.2	830-843 bp	1.0	79.2
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	786-799 bp	1.0	79.2
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	785-798 bp	1.0	79.2
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	820-833 bp	1.0	78.5