Hotspot

petN-psbM

LSC · 28390-28983 · 594 bp

Back to Erythrodes

Pi 0.00515

MarkerSeek score 63.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 594
variable_sites: 3
pi: 0.00515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	752-763 bp	1.0	79.7
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	695-706 bp	1.0	78.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	747-758 bp	1.0	78.2
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	748-759 bp	1.0	77.2
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	CGTTTATTGCTACTGCGCTG	55.0	50.0	57.5	57.9	695-706 bp	1.0	77.1