Hotspot

atpH-atpI

LSC · 13513-14046 · 534 bp

Back to Erythrodes

Pi 0.00794

MarkerSeek score 66.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 4
pi: 0.00794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	603-631 bp	1.0	82.2
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	602-630 bp	1.0	81.7
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.3	57.9	597-625 bp	1.0	81.4
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.9	58.3	597-625 bp	1.0	81.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	GGCTGCGGCTTATATAGGTG	50.0	55.0	57.8	58.3	596-624 bp	1.0	80.6