Hotspot

atpF-atpH

LSC · 12744-13266 · 523 bp

Back to Erythrodes

Pi 0.0189

MarkerSeek score 61.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 8
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AAAACTCCCAACGGATGACC	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.1	58.0	537-637 bp	1.0	84.0
atpF-atpH_p2	AAAACTCCCAACGGATGACC	GGTCGTGGCATTAGCTCTTT	50.0	50.0	58.1	58.3	526-626 bp	1.0	82.3
atpF-atpH_p3	AACTCAAAACTCCCAACGGA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.3	58.0	542-642 bp	1.0	79.0
atpF-atpH_p4	CAACTCAAAACTCCCAACGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.2	58.0	543-643 bp	1.0	78.6
atpF-atpH_p5	TCAAAACTCCCAACGGATGA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.1	58.0	539-639 bp	1.0	77.3