Hotspot

psbK-psbI

LSC · 7382-7838 · 457 bp

Back to Erythrodes

Pi 0.0153
MarkerSeek score 53.80
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
457
variable_sites
7
pi
0.0153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACTACTGTGTAGACGAAGAGT 50.0 42.9 57.9 55.5 522-541 bp 1.0 52.8
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG ACTACTGTGTAGACGAAGAGTT 50.0 40.9 57.9 56.3 522-541 bp 1.0 50.9
psbK-psbI_p3 TGGCAAGCTGCTGTAAGTTT ACTACTGTGTAGACGAAGAGT 45.0 42.9 58.3 55.5 519-538 bp 1.0 50.8
psbK-psbI_p4 TTGGCAAGCTGCTGTAAGTT ACTACTGTGTAGACGAAGAGT 45.0 42.9 58.3 55.5 520-539 bp 1.0 50.8
psbK-psbI_p5 TTTGGCAAGCTGCTGTAAGT ACTACTGTGTAGACGAAGAGT 45.0 42.9 58.3 55.5 521-540 bp 1.0 50.8