Hotspot

trnK-UUU-rps16

LSC · 4324-5009 · 686 bp

Back to Erythrodes

Pi 0.00733

MarkerSeek score 63.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 5
pi: 0.00733
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	CTTGAAAAGGGCGCTCAAC	50.0	52.6	58.2	57.5	796-798 bp	1.0	67.7
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	CTTGAAAAGGGCGCTCAAC	50.0	52.6	58.2	57.5	795-797 bp	1.0	67.7
trnK-UUU-rps16_p3	AGGATCAGTCGCGGTCTTAT	CTTGAAAAGGGCGCTCAAC	50.0	52.6	58.3	57.5	881-883 bp	1.0	66.5
trnK-UUU-rps16_p4	TACCGTTGAGTTAGCAACCC	CTTGAAAAGGGCGCTCAAC	50.0	52.6	57.5	57.5	782-784 bp	1.0	65.4
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	CTTGAAAAGGGCGCTCAAC	50.0	52.6	58.6	57.5	807-809 bp	1.0	64.5