Hotspot

ndhA

SSC · 117259-119516 · 2258 bp

Back to Erythrina

Pi 0.0107

MarkerSeek score 51.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2258
variable_sites: 35
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATGAATATCGAAGGTCCACA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	53.8	56.5	2304-2332 bp	1.0	41.6
ndhA_p2	TGAATATCGAAGGTCCACAT	TGGGAGAAGTTGATCGTTGA	40.0	45.0	53.8	56.5	2303-2331 bp	1.0	41.6
ndhA_p3	TGAATATCGAAGGTCCACATT	TGGGAGAAGTTGATCGTTGA	38.1	45.0	54.7	56.5	2303-2331 bp	1.0	41.6
ndhA_p4	ATGAATATCGAAGGTCCACA	TGGGAGAAGTTGATCGTTGAA	40.0	42.9	53.8	57.2	2304-2332 bp	1.0	41.6
ndhA_p5	TGAATATCGAAGGTCCACAT	TGGGAGAAGTTGATCGTTGAA	40.0	42.9	53.8	57.2	2303-2331 bp	1.0	41.6