Hotspot

ndhI-ndhA

SSC · 117184-117258 · 75 bp

Back to Erythrina

Pi 0.0267

MarkerSeek score 65.30

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 75
variable_sites: 3
pi: 0.0267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	CTCGGACTGTTTGTTGACCA	AGATGGACTTTGCCTAGGCT	50.0	50.0	58.1	58.4	247 bp	1.0	77.3
ndhI-ndhA_p2	CCTATGTACCTTGCAGCTCG	AGATGGACTTTGCCTAGGCT	55.0	50.0	58.1	58.4	263 bp	1.0	76.5
ndhI-ndhA_p3	CTATGTACCTTGCAGCTCGG	AGATGGACTTTGCCTAGGCT	55.0	50.0	58.1	58.4	262 bp	1.0	76.5
ndhI-ndhA_p4	AGCTCGGACTGTTTGTTGAC	AGATGGACTTTGCCTAGGCT	50.0	50.0	58.4	58.4	249 bp	1.0	74.3
ndhI-ndhA_p5	CAGCTCGGACTGTTTGTTGA	AGATGGACTTTGCCTAGGCT	50.0	50.0	58.4	58.4	250 bp	1.0	74.3