Hotspot

rps3-rps19

LSC · 81667-82178 · 512 bp

Back to Erythrina

Pi 0.0268

MarkerSeek score 70.90

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 20
pi: 0.0268
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	TAAACTTCCGAGGACATGCG	50.0	50.0	58.4	58.0	596-602 bp	1.0	82.6
rps3-rps19_p2	CAAGTCTGAAACCAAGCGGA	TCCGAGGACATGCGAAAAAT	50.0	45.0	58.4	57.9	590-596 bp	1.0	81.8
rps3-rps19_p3	CAAGTCTGAAACCAAGCGGA	TTCCGAGGACATGCGAAAAA	50.0	45.0	58.4	58.1	591-597 bp	1.0	81.8
rps3-rps19_p4	AAGTCTGAAACCAAGCGGAT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	595-601 bp	1.0	81.3
rps3-rps19_p5	AGTCTGAAACCAAGCGGATT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	594-600 bp	1.0	81.3