Hotspot

rpl36-rps8

LSC · 77589-78076 · 488 bp

Back to Erythrina

Pi 0.0219

MarkerSeek score 57.20

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 16
pi: 0.0219
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ACGAACAGAGGCTCTTATTTTCA	GGTGGAGAAGTTTTATGCTA	39.1	40.0	58.1	52.6	542-556 bp	1.0	42.3
rpl36-rps8_p2	ACGAACAGAGGCTCTTATTTTCA	GGTGGAGAAGTTTTATGCTAT	39.1	38.1	58.1	53.3	542-556 bp	1.0	42.3
rpl36-rps8_p3	CGAACAGAGGCTCTTATTTTCA	GGTGGAGAAGTTTTATGCTA	40.9	40.0	56.5	52.6	541-555 bp	1.0	42.3
rpl36-rps8_p4	ACGAACAGAGGCTCTTATTTTC	GGTGGAGAAGTTTTATGCTA	40.9	40.0	56.5	52.6	542-556 bp	1.0	42.3
rpl36-rps8_p5	ACGAACAGAGGCTCTTATTT	GGTGGAGAAGTTTTATGCTA	40.0	40.0	54.4	52.6	542-556 bp	1.0	42.3