Hotspot

psbB-psbT

LSC · 71604-71782 · 179 bp

Back to Erythrina

Pi 0.0409

MarkerSeek score 81.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 9
pi: 0.0409
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TTTTTGCTGGTATCGACCCA	TCGTTGGAACTTTAGGAGGT	45.0	45.0	57.4	56.1	363-368 bp	1.0	64.3
psbB-psbT_p2	TTTTTGCTGGTATCGACCCA	TCGTTGGAACTTTAGGAGGTTC	45.0	45.5	57.4	58.0	363-368 bp	1.0	63.6
psbB-psbT_p3	TTTTTGCTGGTATCGACCCA	CGTTGGAACTTTAGGAGGTTCT	45.0	45.5	57.4	58.0	362-367 bp	1.0	63.6
psbB-psbT_p4	TTTTTGCTGGTATCGACCCA	TCGTTGGAACTTTAGGAGGTT	45.0	42.9	57.4	56.9	363-368 bp	1.0	62.5
psbB-psbT_p5	TTTTTGCTGGTATCGACCCA	TTCGTTGGAACTTTAGGAGGT	45.0	42.9	57.4	56.9	364-369 bp	1.0	62.5